Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Spatholobus suberectus (Fabaceae) extract on second-degree burns in rats

Lingyun Dai, Chunhu Zhao, Yu Jian, Zujun Mei, Xufeng Pei, Wensheng Yuan, Fengxue Wu

The Second Clinical Medical College, Yangtze University, Jingzhou City, Hubei Province, 434020, China;

For correspondence:-

Accepted: 16 September 2017 Published: 31 October 2017

Citation: Dai L, Zhao C, Jian Y, Mei Z, Pei X, Yuan W, et al. Effect of Spatholobus suberectus (Fabaceae) extract on second-degree burns in rats. Trop J Pharm Res 2017; 16(10):2365-2371 doi: 10.4314/tjpr.v16i10.8

© 2017 The authors.
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Abstract

Purpose: To evaluate the wound-healing effect of Spatholobus suberectus (Fabaceae) on second-degree burns in a rat model.

Methods: The animals were divided into normal, negative control, as well as 10 % Spatholobus suberectus (SS) (SS10), 20 % SS (SS20) and standard (STD) groups. Second-degree burns were inflicted by exposing a 3 × 3 cm sterile area of skin to boiling water for 10 min. The animals were treated topically twice daily for 2 weeks. Wound contraction (%) was measured after 2 weeks, while wound tissue histopathology was assessed by hematoxylin & eosin and Masson’s trichrome staining. In addition, lipid peroxidation (malondialdehyde kit) and cytokine secretion (ELISA) were measured in liver and plasma, respectively.

Results: The results of this study suggest that topical application of SS for 2 weeks significantly increases wound closure compared with the negative control. Moreover, treatment with SS significantly improved the pathological status of the wound throughout the protocol. There was also a significant decrease in malondialdehyde activity and increase in cytokine release in SS-treated rats compared with control rats.

Conclusions: The results show that topical application of SS after inflicting second-degree burns in rats results in increased wound healing and decreased cytokine release and oxidative stress

Keywords: Spatholobus suberectus, Burns, Wound, Lipid peroxidation, Cytokines